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Ion exchange analysis of synthetic oligonucleotides
Summary
Type:
HPLC
Application #:
107360
Catalog#:
719469 - VA 50/4.6 Nucleogel SAX 1000-8
Detection:
UV, 260nm
Conditions:
eluent A: 0.02M KH2PO4 pH 5.5, 5M urea; eluent B: A + 1M KCl, linear gradient 0 - 100% B in 30 min
flow rate 1ml/min
Authors:
Macherey-Nagel
Source:
LC department
Details
Legend: Separation of synthetic oligonucleotides: 1. 6mer, 2. 24mer, 3. 30mer, 4. 29mer
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